Bio-Protocol (Apr 2017)

Heparan Sulfate Identification and Characterisation: Method I. Heparan Sulfate Identification by NMR Analysis

  • Susan Carnachan,
  • Simon F.R. Hinkley

DOI
https://doi.org/10.21769/bioprotoc.2196
Journal volume & issue
Vol. 7, no. 7

Abstract

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Heparin and heparan sulfate (HS) may be purified from complex biological matrices and are often isolated in sub-milligram quantities but not unequivocally identified by spectroscopic means. This protocol details a methodology to rapidly assess the gross compositional features and approximate purity of HS by 1H nuclear magnetic resonance. A complimentary method for identification and characterisation of heparan sulfate can be found at Carnachan and Hinkley (2017).