Detection the Ability of Aspergillus flavus Isolated from Wheat Grains for Aflatoxin B1 Production using RT- PCR

Abstract

Aspergillus flavus is aflatoxinogenic and potential aflatoxins producers in agricultural commodities. The present study was conducted determine the ability of eleven strains of A. flavus isolated from Iraqi wheat grains Triticum aestivum. The isolates have been detected by molecular methods using Reverse Transcriptase RTPCR. In this study, RNA was extracted from A. flavus, cDNA synthesis and rapid assessment of eleven isolates of A. flavus was accomplished using primer pair for the aflatoxin regulatory gene aflR Reverse transcription-Polymerase chain reaction (RT–PCR).Positive amplification was achieved for all the isolates with a molecular weight 798 to aflR1 and 400bp to aflR2. Also the result of the amplification showed there are no differences with the two molecular weight between the 11 isolated strains of A. flavus in their aflatoxin B1 production, but the first strain differed in their banding florescence as compared with others strains this reflect the genetic differences in aflatoxin B1 production between them.

Keywords

• AflatoxinsB1, Aspergillus flavus, RT–PCR