Gene frequency and distribution of human platelet antigen-1 to −6, −10, −15, and −21 of platelets donors in Wuhan, China

Abstract

Background: In this study, the frequency of human platelet antigen (HPA) alleles and genotypes of platelet donors in Wuhan was evaluated and differences in HPA distribution between Wuhan and other regions in China were compared. Study Design and Methods: HPA genotyping was performed using RT-qPCR in 1516 platelet donors in Wuhan. The allele frequencies of HPA-1 to −6, −10, −15, and −21 were calculated and evaluated according to the Hardy–Weinberg principle. Mismatch probability was calculated for each HPA system. The distribution and features of HPA systems in Wuhan were compared to those of other regions in China. Results: The observed allele frequencies and expected frequencies of HPA-1 to −6, −10, −15, and −21 showed no significant differences according to the Hardy–Weinberg principle (all P > 0.05). The prevalent allele among the nine HPA systems assessed in this study was allele “a”, except in the HPA-3 and HPA-15, where allele “b” was found in 43.3 % and 46.9 % of platelet donors, respectively. The highest transfusion mismatch probability was found in HPA-15 (37.40 %) and HPA-3 (37.05 %). Compared to other regions of China, Wuhan exhibits distinct characteristics in the distribution of HPA allele frequencies and genotypes among platelet donors. Conclusion: Distinct gene frequencies and genotypes were observed for HPA-1 to −6, −10, −15, and −21 among 1516 platelet donors from Wuhan compared with those of other regions. These results highlight the fact that HPA genotyping is of great significance for the establishment of HPA genotype registry and precise platelet transfusion in Wuhan.

Keywords

• Human platelet antigen
• Genotyping
• Gene frequency