Tumor-derived extracellular vesicles as a biomarker for breast cancer diagnosis and metastasis monitoring
Feng Xu,
Ke Wang,
Chengjun Zhu,
Lingling Fan,
Ye Zhu,
Jacqueline F. Wang,
Xintong Li,
Yiqiu Liu,
Yuhan Zhao,
Chuandong Zhu,
Wenwen Zhang,
Fang Yang,
Jian Xu,
Zhe Li,
Xiaoxiang Guan
Affiliations
Feng Xu
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
Ke Wang
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
Chengjun Zhu
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
Lingling Fan
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
Ye Zhu
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
Jacqueline F. Wang
Department of Medicine, NYU Langone Health, 550 First Avenue, New York 10016, NY, USA
Xintong Li
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
Yiqiu Liu
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
Yuhan Zhao
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
Chuandong Zhu
Department of Oncology, Nanjing Second Hospital, Nanjing, China
Wenwen Zhang
Department of Oncology, Nanjing First Hospital, Nanjing, China
Fang Yang
The Comprehensive Cancer Center of Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing , China
Jian Xu
Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China; Corresponding author
Zhe Li
Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing University, Nanjing, China; State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, Nanjing, China; Corresponding author
Xiaoxiang Guan
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China; Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Collaborative Innovation Center for Personalized Cancer Medicine, Nanjing Medical University, Nanjing, China; Corresponding author
Summary: It is imperative to explore biomarkers that are both precise and readily accessible in the comprehensive management of breast cancer. A multicenter cohort, including 512 breast cancer patients and 198 nonneoplastic individuals, was recruited to detect the level of tumor-derived extracellular vesicles using our method based on dual DNA tetrahedral nanostructures. The level of tumor-derived extracellular vesicles was significantly higher in newly diagnosed breast cancer patients than in nonneoplastic individuals at a cutoff value of 3.58 U/μL. For postoperative metastasis monitoring, the level of tumor-derived extracellular vesicles was significantly higher in breast cancer patients with metastasis than in those without metastasis at a cutoff value of 3.91 U/μL. Its efficacy of diagnosis and metastasis monitoring was superior to traditional tumor markers. Elevated level of tumor-derived extracellular vesicles served as a predictive biomarker for diagnosis and metastasis monitoring in breast cancer patients.
