Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)

Liliana Barbieri; Huw Colin-York; Kseniya Korobchevskaya; Di Li; Deanna L. Wolfson; Narain Karedla; Falk Schneider; Balpreet S. Ahluwalia; Tore Seternes; Roy A. Dalmo; Michael L. Dustin; Dong Li; Marco Fritzsche

doi:10.1038/s41467-021-22377-9

Nature Communications (Apr 2021)

Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)

Liliana Barbieri,
Huw Colin-York,
Kseniya Korobchevskaya,
Di Li,
Deanna L. Wolfson,
Narain Karedla,
Falk Schneider,
Balpreet S. Ahluwalia,
Tore Seternes,
Roy A. Dalmo,
Michael L. Dustin,
Dong Li,
Marco Fritzsche

Affiliations

Liliana Barbieri: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford
Huw Colin-York: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford
Kseniya Korobchevskaya: Kennedy Institute for Rheumatology, University of Oxford
Di Li: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Deanna L. Wolfson: Department of Physics and Technology, UiT The Arctic University of Norway
Narain Karedla: Kennedy Institute for Rheumatology, University of Oxford
Falk Schneider: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford
Balpreet S. Ahluwalia: Department of Physics and Technology, UiT The Arctic University of Norway
Tore Seternes: Norwegian College of Fishery Science, UiT The Arctic University of Norway
Roy A. Dalmo: Norwegian College of Fishery Science, UiT The Arctic University of Norway
Michael L. Dustin: Kennedy Institute for Rheumatology, University of Oxford
Dong Li: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Marco Fritzsche: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford

DOI: https://doi.org/10.1038/s41467-021-22377-9
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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Quantifying rapid and small cellular forces is a major challenge in mechanobiology. Here, the authors show a >2-fold spatially and >10-fold temporally force sampling improvement combining traction force microscopy with total internal reflection fluorescence super-resolution structured illumination microscopy.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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