Frontiers in Bioengineering and Biotechnology (Feb 2020)

Metabolic Engineering of Bacillus licheniformis for Production of Acetoin

  • Chuanjuan Lü,
  • Yongsheng Ge,
  • Menghao Cao,
  • Xiaoting Guo,
  • Peihai Liu,
  • Chao Gao,
  • Ping Xu,
  • Cuiqing Ma

DOI
https://doi.org/10.3389/fbioe.2020.00125
Journal volume & issue
Vol. 8

Abstract

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Acetoin is a potential platform compound for a variety of chemicals. Bacillus licheniformis MW3, a thermophilic and generally regarded as safe (GRAS) microorganism, can produce 2,3-butanediol with a high concentration, yield, and productivity. In this study, B. licheniformis MW3 was metabolic engineered for acetoin production. After deleting two 2,3-butanediol dehydrogenases encoding genes budC and gdh, an engineered strain B. licheniformis MW3 (ΔbudCΔgdh) was constructed. Using fed-batch fermentation of B. licheniformis MW3 (ΔbudCΔgdh), 64.2 g/L acetoin was produced at a productivity of 2.378 g/[L h] and a yield of 0.412 g/g from 156 g/L glucose in 27 h. The fermentation process exhibited rather high productivity and yield of acetoin, indicating that B. licheniformis MW3 (ΔbudCΔgdh) might be a promising acetoin producer.

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