Microfluidics delivery of DARPP-32 into HeLa cells maintains viability for in-cell NMR spectroscopy

Nicholas Sciolino; Anna Liu; Leonard Breindel; David S. Burz; Todd Sulchek; Alexander Shekhtman

doi:10.1038/s42003-022-03412-x

Communications Biology (May 2022)

Microfluidics delivery of DARPP-32 into HeLa cells maintains viability for in-cell NMR spectroscopy

Nicholas Sciolino,
Anna Liu,
Leonard Breindel,
David S. Burz,
Todd Sulchek,
Alexander Shekhtman

Affiliations

Nicholas Sciolino: University at Albany, Department of Chemistry
Anna Liu: Georgia Tech, School of Mechanical Engineering
Leonard Breindel: University at Albany, Department of Chemistry
David S. Burz: University at Albany, Department of Chemistry
Todd Sulchek: Georgia Tech, School of Mechanical Engineering
Alexander Shekhtman: University at Albany, Department of Chemistry

DOI: https://doi.org/10.1038/s42003-022-03412-x
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 8

Abstract

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The microfluidic technique of cell volume exchange for convective transfer, VECT can be used to deliver the DARPP-32 protein into cells for in-cell NMR experiments.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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