THE AUGMENTATION OF PLATELET-RICH FIBRIN TO OSTEOCALCIN AND HISTOPATHOLOGICAL SCORE OF BONE HEALING IN BONE DEFECT MODEL RATS SUPPLEMENTED WITH BONE AUTOGRAFT

Abstract

Platelet-rich fibrin (PRF), is a biomaterial that promotes soft tissue and bone healing. In a long bone defect rat model, the current study compares the effectiveness of bone autograft with PRF augmentation versus a standard bone graft procedure. There are two groups in this study, and the control group is limited to the post-test. There were 7 rats in each control (CG) and intervention group (IG). After the making of the autologous PRF, the intervention group received an autologous bone graft and PRF, as the control group only received a bone graft from the iliac crest. The graft was maintained for 4 weeks. Selected bone was chosen for immunohistochemistry of osteocalcin and histopathological measurement of bone healing. There was a statistically significant increase in osteocalcin expression compared to control in the cell cytoplasmic (p=0.040) and the osteoid matrix (p=0.025). Compared to the control group, there were no statistically significant increases in the nucleus's expression of osteocalcin in the intervention group (p=0.067). The intervention group had a clinically significant better histopathological score of bone healing (p=0.015). This study shows the effect of PRF as a biomaterial that can enhance the new bone formation process in the bone defect. This effect could be a result due to the fibrin mesh of PRF could protect the GF from proteolysis. The application of PRF combined with autologous bone graft could increase the expression of osteocalcin and increase the formation of new bone tissue in rats with a long bone defect model.

Keywords

• prf
• osteocalcin
• immunohistochemistry
• bone defect