Extraction of Chitin and Chitosan from Wild Type Pleurotus Spp and its Potential Application - Innovative Approach

Abstract

Biopolymer of chitin and chitosan were extracted from the two Pleurotus spp. of P.floridanus and P.djamor. After the optimization, genomic DNA was isolated, and by using ITS 1 and ITS 4 primers molecular identification was carried out and the nucleotides were submitted to NCBI. The biopolymer have a great commercial importance now a days because of it high efficiency in all the field. The present study was focused on to extract the chitin and chitosan from the Pleurotus spp. and these polymer was subjected to analysis the total antioxidant activity, antimicrobial activity against food borne pathogens of E.coli, B. cereus, S.aureus, A.niger and A.flavus. Cytotoxicity study against HeLa Cell Line was also performed to study the efficacy of the extracted polymer.

Keywords

• pleurotus spp
• chitin
• chitosan
• total antioxidant
• antimicrobial activity
• cytotoxicity