High-efficiency RNA-based reprogramming of human primary fibroblasts

Igor Kogut; Sandra M. McCarthy; Maryna Pavlova; David P. Astling; Xiaomi Chen; Ana Jakimenko; Kenneth L. Jones; Andrew Getahun; John C. Cambier; Anna M. G. Pasmooij; Marcel F. Jonkman; Dennis R. Roop; Ganna Bilousova

doi:10.1038/s41467-018-03190-3

Nature Communications (Feb 2018)

High-efficiency RNA-based reprogramming of human primary fibroblasts

Igor Kogut,
Sandra M. McCarthy,
Maryna Pavlova,
David P. Astling,
Xiaomi Chen,
Ana Jakimenko,
Kenneth L. Jones,
Andrew Getahun,
John C. Cambier,
Anna M. G. Pasmooij,
Marcel F. Jonkman,
Dennis R. Roop,
Ganna Bilousova

Affiliations

Igor Kogut: Department of Dermatology, University of Colorado School of Medicine
Sandra M. McCarthy: Department of Dermatology, University of Colorado School of Medicine
Maryna Pavlova: Department of Dermatology, University of Colorado School of Medicine
David P. Astling: Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine
Xiaomi Chen: Department of Dermatology, University of Colorado School of Medicine
Ana Jakimenko: Department of Dermatology, University of Colorado School of Medicine
Kenneth L. Jones: Department of Pediatrics, University of Colorado School of Medicine
Andrew Getahun: Department of Immunology and Microbiology, University of Colorado School of Medicine
John C. Cambier: Department of Immunology and Microbiology, University of Colorado School of Medicine
Anna M. G. Pasmooij: Department of Dermatology, University Medical Center
Marcel F. Jonkman: Department of Dermatology, University Medical Center
Dennis R. Roop: Department of Dermatology, University of Colorado School of Medicine
Ganna Bilousova: Department of Dermatology, University of Colorado School of Medicine

DOI: https://doi.org/10.1038/s41467-018-03190-3
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 15

Abstract

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Induced pluripotent stem cells (iPSCs) have potential for regenerative medicine applications, but are generated with very low efficiency. Here, the authors show highly efficient reprogramming of human primary fibroblasts to iPSCs via the synergistic activity of synthetic modified mRNAs, mature miRNA mimics, and optimized culture methods.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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