Background Noise Suppression of Optical Sectioning Structured Illumination Microscopy via Fourier Domain Reconstruction

Shipei Dang; Shipei Dang; Jia Qian; Tong Peng; Chen Bai; Junwei Min; Haixia Wang; Baoli Yao; Baoli Yao; Dan Dan

doi:10.3389/fphy.2022.900686

Frontiers in Physics (Apr 2022)

Background Noise Suppression of Optical Sectioning Structured Illumination Microscopy via Fourier Domain Reconstruction

Shipei Dang,
Shipei Dang,
Jia Qian,
Tong Peng,
Chen Bai,
Junwei Min,
Haixia Wang,
Baoli Yao,
Baoli Yao,
Dan Dan

Affiliations

Shipei Dang: State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an, China
Shipei Dang: University of Chinese Academy of Sciences, Beijing, China
Jia Qian: State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an, China
Tong Peng: State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an, China
Chen Bai: State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an, China
Junwei Min: State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an, China
Haixia Wang: The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
Baoli Yao: State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an, China
Baoli Yao: University of Chinese Academy of Sciences, Beijing, China
Dan Dan: State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an, China

DOI: https://doi.org/10.3389/fphy.2022.900686
Journal volume & issue: Vol. 10

Abstract

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Optical sectioning structured illumination microscopy (OS-SIM) has been attracting considerable interest in fast 3D microscopy. The reconstruction of optical sectioning images in the conventional method employs the root-mean-square (RMS) algorithm in the spatial domain, which is prone to residual background noise. To overcome this problem, we propose a Fourier domain based optical sectioning image reconstruction algorithm (termed Fourier-OS-SIM), which has an improved background noise suppression capability compared to the RMS algorithm. The experimental results verified the feasibility and the effectiveness of the algorithm. The improved performance of the Fourier-OS-SIM may find more applications in biomedical or industrial fields.

Published in Frontiers in Physics

ISSN: 2296-424X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Physics
Website: https://www.frontiersin.org/journals/physics

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