Rutin-Functionalized Multi-Walled Carbon Nanotubes: Molecular Docking, Physicochemistry and Cytotoxicity in Fibroblasts

Conrado M. S. Neto; Felipe C. Lima; Renata P. Morais; Lucas R. M. de Andrade; Renata de Lima; Marco V. Chaud; Matheus M. Pereira; Ricardo L. C. de Albuquerque Júnior; Juliana C. Cardoso; Aleksandra Zielińska; Eliana B. Souto; Álvaro S. Lima; Patrícia Severino

doi:10.3390/toxics9080173

Toxics (Jul 2021)

Rutin-Functionalized Multi-Walled Carbon Nanotubes: Molecular Docking, Physicochemistry and Cytotoxicity in Fibroblasts

Conrado M. S. Neto,
Felipe C. Lima,
Renata P. Morais,
Lucas R. M. de Andrade,
Renata de Lima,
Marco V. Chaud,
Matheus M. Pereira,
Ricardo L. C. de Albuquerque Júnior,
Juliana C. Cardoso,
Aleksandra Zielińska,
Eliana B. Souto,
Álvaro S. Lima,
Patrícia Severino

Affiliations

Conrado M. S. Neto: Institute of Technology and Research (ITP), University of Tiradentes (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
Felipe C. Lima: Institute of Technology and Research (ITP), University of Tiradentes (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
Renata P. Morais: Institute of Technology and Research (ITP), University of Tiradentes (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
Lucas R. M. de Andrade: Institute of Technology and Research (ITP), University of Tiradentes (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
Renata de Lima: School of Bioprocess Engineering and Biotechnology, Sorocaba University, Rodovia Raposo Tavares km 92, Sorocaba 18023-000, São Paulo, Brazil
Marco V. Chaud: School of Bioprocess Engineering and Biotechnology, Sorocaba University, Rodovia Raposo Tavares km 92, Sorocaba 18023-000, São Paulo, Brazil
Matheus M. Pereira: Department of Chemistry, CICECO—Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, Portugal
Ricardo L. C. de Albuquerque Júnior: Institute of Technology and Research (ITP), University of Tiradentes (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
Juliana C. Cardoso: Institute of Technology and Research (ITP), University of Tiradentes (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
Aleksandra Zielińska: Institute of Human Genetics, Polish Academy of Sciences Poznan, 60-479 Poznan, Poland
Eliana B. Souto: Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal
Álvaro S. Lima: Institute of Technology and Research (ITP), University of Tiradentes (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
Patrícia Severino: Institute of Technology and Research (ITP), University of Tiradentes (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil

DOI: https://doi.org/10.3390/toxics9080173
Journal volume & issue: Vol. 9, no. 8
p. 173

Abstract

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Multi-Walled Carbon Nanotubes (MWCNT) have been functionalized with rutin through three steps (i. reaction step; ii. purification step; iii. drying step) and their physicochemical properties investigated with respect to morphological structure, thermal analysis, Fourier Transform Infrared Spectroscopy (FTIR), and cytotoxicity. The molecular docking suggested the rutin-functionalized MWCNT occurred by hydrogen bonds, which was confirmed by FTIR assays, corroborating the results obtained by thermal analyses. A tubular shape, arranged in a three-dimensional structure, could be observed. Mild cytotoxicity observed in 3T3 fibroblasts suggested a dose–effect relationship after exposure. These findings suggest the formation of aggregates of filamentous structures on the cells favoring the cell penetration.

Published in Toxics

ISSN: 2305-6304 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology
Website: http://www.mdpi.com/journal/toxics/

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