Frontiers in Plant Science (Dec 2021)
Re-examination of the APETALA2/Ethylene-Responsive Factor Gene Family in Barley (Hordeum vulgare L.) Indicates a Role in the Regulation of Starch Synthesis
- Jinjin Ding,
- Jinjin Ding,
- Hassan Karim,
- Hassan Karim,
- Yulong Li,
- Yulong Li,
- Wendy Harwood,
- Carlos Guzmán,
- Na Lin,
- Qiang Xu,
- Qiang Xu,
- Yazhou Zhang,
- Yazhou Zhang,
- Huaping Tang,
- Huaping Tang,
- Yunfeng Jiang,
- Yunfeng Jiang,
- Pengfei Qi,
- Pengfei Qi,
- Mei Deng,
- Mei Deng,
- Jian Ma,
- Jian Ma,
- Jirui Wang,
- Jirui Wang,
- Guoyue Chen,
- Guoyue Chen,
- Xiujin Lan,
- Xiujin Lan,
- Yuming Wei,
- Yuming Wei,
- Youliang Zheng,
- Youliang Zheng,
- Qiantao Jiang,
- Qiantao Jiang
Affiliations
- Jinjin Ding
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Jinjin Ding
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Hassan Karim
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Hassan Karim
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Yulong Li
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Yulong Li
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Wendy Harwood
- John Innes Center, Norwich Research Park, Norwich, United Kingdom
- Carlos Guzmán
- Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, Córdoba, Spain
- Na Lin
- College of Sichuan Tea, Yibin University, Yibin, China
- Qiang Xu
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Qiang Xu
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Yazhou Zhang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Yazhou Zhang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Huaping Tang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Huaping Tang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Yunfeng Jiang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Yunfeng Jiang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Pengfei Qi
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Pengfei Qi
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Mei Deng
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Mei Deng
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Jian Ma
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Jian Ma
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Jirui Wang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Jirui Wang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Guoyue Chen
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Guoyue Chen
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Xiujin Lan
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Xiujin Lan
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Yuming Wei
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Yuming Wei
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Youliang Zheng
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Youliang Zheng
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Qiantao Jiang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Qiantao Jiang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- DOI
- https://doi.org/10.3389/fpls.2021.791584
- Journal volume & issue
-
Vol. 12
Abstract
The APETALA2/Ethylene-Responsive factor (AP2/ERF) gene family is a large plant-specific transcription factor family, which plays important roles in regulating plant growth and development. A role in starch synthesis is among the multiple functions of this family of transcription factors. Barley (Hordeum vulgare L.) is one of the most important cereals for starch production. However, there are limited data on the contribution of AP2 transcription factors in barley. In this study, we used the recently published barley genome database (Morex) to identify 185 genes of the HvAP2/ERF family. Compared with previous work, we identified 64 new genes in the HvAP2/ERF gene family and corrected some previously misannotated and duplicated genes. After phylogenetic analysis, HvAP2/ERF genes were classified into four subfamilies and 18 subgroups. Expression profiling showed different patterns of spatial and temporal expression for HvAP2/ERF genes. Most of the 12 HvAP2/ERF genes analyzed using quantitative reverse transcription–polymerase chain reaction had similar expression patterns when compared with those of starch synthase genes in barley, except for HvAP2-18 and HvERF-73. HvAP2-18 is homologous to OsRSR1, which negatively regulates the synthesis of rice starch. Luciferase reporter gene, and yeast one-hybrid assays showed that HvAP2-18 bound the promoter of AGP-S and SBE1 in vitro. Thus, HvAP2-18 might be an interesting candidate gene to further explore the mechanisms involved in the regulation of starch synthesis in barley.
Keywords
