ИССЛЕДОВАНИЕ ЭФФЕКТИВНОСТИ ИНДУКЦИИ ЦИТОТОКСИЧЕСКОГО ИММУННОГО ОТВЕТА МОНОНУКЛЕАРНЫМИ КЛЕТКАМИ С ПОМОЩЬЮ ДЕНДРИТНЫХ КЛЕТОК, ТРАНСФЕЦИРОВАННЫХ ПОЛИЭПИТОПНЫМИ КОНСТРУКЦИЯМИ HER2/ERBB2

Abstract

This study describes a design of genetic constructs encoding either non-specific, or HLA-A *0201-specific polyepitopic immunogens containing antigenic determinants for HER2 protein. We have shown that transfection of mature dendritic cells by polyepitopic constructs with magnetic nanoparticles lead to effective in vitro stimulation of cytotoxic immune response of mononuclear cell populations obtained from HLA-A0201-positive healthy donors/ This cytotoxic action was assessed in vitro, as lethal effects upon HLA-A0201/HER2 double positive MCF-7 tumor cells, and expression of perforin granules.

Keywords

• cytotoxicity
• polyepitopic constructs
• dendritic cells
• transfection