Molecular cloning, identification, and expression analysis of Pyy and its effect on mechanisms regulating appetite in Spotted scat, Scatophagus argus

Daniel Assan; Umar Farouk Mustapha; Charles Brighton Ndandala; Zhiyuan Li; Guangli Li; Chunren Huang; Guangwen Zhao; Huapu Chen; Mouyan Jiang

Aquaculture Reports (Feb 2023)

Molecular cloning, identification, and expression analysis of Pyy and its effect on mechanisms regulating appetite in Spotted scat, Scatophagus argus

Daniel Assan,
Umar Farouk Mustapha,
Charles Brighton Ndandala,
Zhiyuan Li,
Guangli Li,
Chunren Huang,
Guangwen Zhao,
Huapu Chen,
Mouyan Jiang

Affiliations

Daniel Assan: Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Guangdong Province Famous Fish Reproduction and Breeding Engineering Technology Research Center, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China
Umar Farouk Mustapha: Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Guangdong Province Famous Fish Reproduction and Breeding Engineering Technology Research Center, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China
Charles Brighton Ndandala: Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Guangdong Province Famous Fish Reproduction and Breeding Engineering Technology Research Center, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China
Zhiyuan Li: Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Guangdong Province Famous Fish Reproduction and Breeding Engineering Technology Research Center, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China
Guangli Li: Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Guangdong Province Famous Fish Reproduction and Breeding Engineering Technology Research Center, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources of Ministry of Education, Hainan Key Laboratory for Conservation and Utilization of Tropical Marine Fishery Resources, Yazhou Bay Innovation Institute, Hainan Tropical Ocean University, Sanya 572022, China
Chunren Huang: Hainan Chenhai Aquatic co., LTD, Sanya 572022, China
Guangwen Zhao: Hainan Chenhai Aquatic co., LTD, Sanya 572022, China
Huapu Chen: Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Guangdong Province Famous Fish Reproduction and Breeding Engineering Technology Research Center, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources of Ministry of Education, Hainan Key Laboratory for Conservation and Utilization of Tropical Marine Fishery Resources, Yazhou Bay Innovation Institute, Hainan Tropical Ocean University, Sanya 572022, China
Mouyan Jiang: Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Guangdong Province Famous Fish Reproduction and Breeding Engineering Technology Research Center, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Correspondence to: Guangdong Ocean University, No. 1 Haida Road, Mazhang District, Zhanjiang 524088, China.

Journal volume & issue: Vol. 28
p. 101465

Abstract

Read online

The peptide YY (Pyy) is a member of the Neuropeptide Y family of peptides known to play anorexigenic roles in the regulation of feed intake in several fishes. In this study, to examine the role of Pyy in the spotted scat (Scatophagus argus), the peptide was cloned from this fish for the first time. The peptide is made from a 294-bp open reading frame with a 97-amino-acid sequence that is highly conserved when compared to other organisms. The pyy gene is expressed widely throughout the spotted scat, with the highest levels in the intestine and muscle tissue. According to our pre-and post-prandial experiments, feeding has a minimal short-term effect on pyy mRNA levels. In comparison to fish that were refed on the 8th day after 7 days of starvation, pyy mRNA levels in the hypothalamus were considerably higher after 7 days of feeding. Results from this study also identified a local interaction between Pyy and some appetite-regulating peptides in spotted scat. We aimed to build a solid foundation for future research into feed intake regulation of the spotted scat, as this is one of the most cultured species.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

About the journal

Abstract

Keywords