Development of powdery mildew resistant derivatives of wheat variety Fielder for use in genetic transformation
Xiaona Liang,
Xiaomin Bie,
Yuliang Qiu,
Ke Wang,
Zujun Yang,
Yuqi Jia,
Zhiying Xu,
Mei Yu,
Lipu Du,
Zhishan Lin,
Xingguo Ye
Affiliations
Xiaona Liang
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Xiaomin Bie
College of Life Science, Shandong Agricultural University, Tai’an 271018, Shandong, China
Yuliang Qiu
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Ke Wang
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Zujun Yang
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, Sichuan, China
Yuqi Jia
College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China
Zhiying Xu
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Mei Yu
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Lipu Du
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Zhishan Lin
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing 100081, China; Corresponding authors.
Xingguo Ye
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Key Laboratory of Ministry of Agriculture and Rural Affairs of China for Biology and Genetic Breeding of Triticeae Crops, Beijing 100081, China; Corresponding authors.
Genetic transformation is widely used to improve target traits and to study gene function in wheat. However, transformation efficiency depends on the physiological status of the recipient genotype and that is affected by several factors including powdery mildew (PM) infection. The widely used recipient variety Fielder is very susceptible to PM. Therefore, it would be beneficial to develop PM resistant derivatives with high regeneration ability for use in genetic transformation. In the present study PM resistant lines CB037 and Pm97033 carrying genes Pm21 and PmV, respectively, were backcrossed to Fielder with selection for PM resistance. Five lines, NT89, NT90, NT154, and WT48 with Pm21 and line FL347 with PmV were developed, identified by molecular markers and genomic in situ hybridization (GISH) or fluorescent in situ hybridization (FISH), and further subjected to detailed assessment of agronomic traits and regeneration ability following genetic transformation capacity. Lines FL347, WT48, NT89 and NT154 assessed as being equal to, or superior, to Fielder in regeneration and transformation ability are recommended as suitable materials for the replacement of Fielder for wheat gene transfer and genome editing study.
