Фармация и фармакология (Пятигорск) (Jan 2020)
Сerebroprotective effect of some phenolic acids under conditions of experimental brain ischemia
Abstract
The aim of the study was to evaluate the cerebroprotective effect of some phenolic acids under the conditions of experimental cerebral ischemia in rats.Materials and methods. The experiment was conducted on male Wistar rats weighing 220–240 g. Focal cerebral ischemia was modeled by irreversible right-sided thermocoagulation of the middle cerebral artery under chloral hydrate anesthesia (350 mg/kg, intraperitoneally). The experimental compounds (4-hydroxy-3.5-di-tert-butyl cinnamic acid, caffeic acid and gallic acid 100 mg/kg each compound) and a reference drug (Mexicor – 100 mg/kg) were administered intragastrically next day after the surgery and then for three daysrunning. The effect of the test-compounds on the cognitive functions of the rats was evaluated by CRPA and TEA tests. The influence of the compounds on the changes in the concentration of lactate, pyruvate, homocysteine, as well as the degree of cerebral edema formation and necrosis of the brain tissue, were studied.Results. In the study, it has been established that against the background of the focal cerebral ischemia, the administration of caffeic, 4-hydroxy-3,5-di-tert-butylcinnamic and gallic acid, contributed to the preservation of a memorable trace in rats, as well as a decrease in lactate concentration (by 40.37% (p<0.05), 151.26% (p<0.05), 48.02% (p<0.05)) and pyruvate (by 96.6,% (p<0.05), 38, 78% (p<0.05), 33.3% (p<0.05)), homocysteine (by 59.6% (p<0.05), 102.18% (p<0.05), 28.8% (p<0.05)), аnecrosis zone (by 122.79% (p<0.05), 165.11% (p<0.05), 12.38% (p<0,05)) and cerebral edema (by 10.47% (p<0.05), 11.08% (p<0.05), 9.92% (p<0.05)) relative to the NC group of rats.Conclusion. The obtained data indicate the possibility of further detailed investigation of the cerebroprotective effect of 4-hydroxy-3,5-di-tert-butylcinnamic, caffeic and gallic acids.
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