Antibacterial activity of dialkyl-aginate biosurfactant cream againts Staphylococcus aureus an Pseudomonas aerugynosa

Abstract

Dialkyl-alginate biosurfactant is an amphifilic rhamnolipid biosurfactant which has the potential to be developed into an antibacterial agent. The purpose of this study was to prove the biosurfactant of dialkyl alginate and it’s cream formula as antibacterial activity especially against Staphylococcus aureus and Pseudomonas aeruginosa. The antibacterial activity assay of biosurfactant dialkyl alginate at concentrations of 5%, 10% and 20% againts Staphylococcus aureus and Pseudomonas aeruginosa using modified quantitative method. Enbatic® 1% is used as a positive control and sterile aquadest as a negative control. Determination of antibacterial activity of dialkyl alginate biosurfactant followed by analysis of leakage of protein and nucleic acids using UV-Vis Spectrophotometry and leakage of Ca2+ and K + metal ions using Atomic Absorption Spetrophotometry (AAS). The most active concentration was formulated into cream and tested antibacterial activity using the well method. The test results showed that the biosurfactants of dialkyl-alginate and it’s cream formula has antibacterial activity. The concentration of 10% was the most active concentration having activity which did not differ significantly to positive control with p value of 0,05.

Keywords

• antibacterial activity
• alginate
• biosurfactant
• cell leakage
• sargassum sp