Antagonism of <i>Bacillus subtilis </i>strain AG1 against vine wood fungal pathogens

Abstract

Antagonistic substances produced by a Bacillus subtilis strain (AG1), which were previously found to slow down the growth of esca fungi in vitro, were produced in an artificial medium, isolated from the cell-free medium by precipitation and acidification (to less than pH 2.5) and extracted from the precipitate with 96% ethanol. The crude extract employed in antibiotic assays confirmed, in vitro, the antagonism of B. subtilis against Phaeoacremonium aleophilum and Phaeomoniella chlamydospora, and also showed an antifungal activity toward Verticillium dahliae and Botryosphaeria rhodina.