Determination of the Effective Time of Denture Cleanser Tablets on the Removal of Candida Albicans on Denture Base Resins

Abstract

Objective: The aim of this study was to determinate of the effective time of different denture cleanser tablets on the removal of Candida albicans on various base resins. Materials and Methods: Conventional heat cured resin, high impact resin, autopolymerized resin, and polyamide resin were used. 160 samples were prepared for each resin type. The biofilm of C. albicans was formed on the resins and then exposed to alkaline peroxide tablet and enzymatic tablets for 3-5-10-20-40-80-160-200 minutes. Cell viability was assessed by MTT test. 3-way ANOVA was used for statistical analysis. Results: The effect of resin type, tablet type and application time on cell viability of C. albicans were found to be significant (p<0.05). Cell death was at least 65% on the resins even at the minimum time. Furthermore, as the duration of administration of both tablets increased, cell viability in all resins tended to decrease. The administration of both tablets on all resins for 10 min resulted in approximately 80% cell death. Additionally, the most significant antimicrobial activities of the tablets were determined on 20th minutes. The alkaline peroxide tablet on all base resins for application periods of 3 to 200 minutes was generally more effective than the enzymatic tablet. Conclusion: Alkaline peroxide and enzymatic cleanser tablets showed remarkable anticandidal activity for all resins. The patients with risk of C. albicans infection should keep their prostheses in cleanser tablets for at least 20 minutes. Furthermore, the anticandidal effect tended to increase with prolonged exposure time.

Keywords

• denture bases
• candida albicans
• cell survival
• denture cleansers
• protez kaideleri
• kandida albicans
• hücre canlılığı
• protez temizleyicileri̇