A histone H3K4me1-specific binding protein is required for siRNA accumulation and DNA methylation at a subset of loci targeted by RNA-directed DNA methylation

Qingfeng Niu; Zhe Song; Kai Tang; Lixian Chen; Lisi Wang; Ting Ban; Zhongxin Guo; Chanhong Kim; Heng Zhang; Cheng-Guo Duan; Huiming Zhang; Jian-Kang Zhu; Jiamu Du; Zhaobo Lang

doi:10.1038/s41467-021-23637-4

Nature Communications (Jun 2021)

A histone H3K4me1-specific binding protein is required for siRNA accumulation and DNA methylation at a subset of loci targeted by RNA-directed DNA methylation

Qingfeng Niu,
Zhe Song,
Kai Tang,
Lixian Chen,
Lisi Wang,
Ting Ban,
Zhongxin Guo,
Chanhong Kim,
Heng Zhang,
Cheng-Guo Duan,
Huiming Zhang,
Jian-Kang Zhu,
Jiamu Du,
Zhaobo Lang

Affiliations

Qingfeng Niu: Shanghai Center for Plant Stress Biology, National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences
Zhe Song: Shanghai Center for Plant Stress Biology, National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences
Kai Tang: Department of Horticulture and Landscape Architecture, Purdue University
Lixian Chen: Shanghai Center for Plant Stress Biology, National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences
Lisi Wang: Shanghai Center for Plant Stress Biology, National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences
Ting Ban: Shanghai Center for Plant Stress Biology, National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences
Zhongxin Guo: Vector-borne Virus Research Center, College of Plant Protection, Fujian Agriculture and Forestry Universtiy
Chanhong Kim: Shanghai Center for Plant Stress Biology, National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences
Heng Zhang: Shanghai Center for Plant Stress Biology, National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences
Cheng-Guo Duan: Shanghai Center for Plant Stress Biology, National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences
Huiming Zhang: Shanghai Center for Plant Stress Biology, National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences
Jian-Kang Zhu: Shanghai Center for Plant Stress Biology, National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences
Jiamu Du: Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Institute of Plant and Food Science, School of Life Science, Southern University of Science and Technology
Zhaobo Lang: Shanghai Center for Plant Stress Biology, National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences

DOI: https://doi.org/10.1038/s41467-021-23637-4
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 13

Abstract

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In plants, RNA-directed DNA methylation (RdDM) is a de novo DNA methylation pathway that is responsible for transcriptional silencing of repetitive elements. Here, the authors characterized a new RdDM factor, RDM15, and show that it is required for RdDM-dependent DNA methylation and siRNA accumulation at a subset of RdDM target loci.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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