Silver Nanoparticles Synthesis Using Artemisia turcomanica Ethanolic Extract and Study of Anti-proliferative Effects on Cell Line of Gastric Cancer

Abstract

Background: The use of plants for the synthesis of nanoparticles, because of not using pollutants and toxins, is considered an eco-friendly method. Objective: The purpose of this study is silver nanoparticles biosynthesis using Artemisia turcomanica extract and evaluating the toxicity of synthesized silver nanoparticles on gastric cancer cell line (AGS). Methods: For synthesizing silver nanoparticles, the leaf extract of Artemisia turcomanica was used. Phytomorphology of bio silver nanoparticles using Transmission Electron Microscopy (TEM) and Scanning Electron Microscope (SEM) analysis was evaluated. Cytotoxicity effects of bio nanoparticles in two cell lines of gastric cancer (AGS) and fibroblast normal cell (L-929) in concentrations of 3, 5, 12.5, 25, 50, and 100 µg/ml were evaluated in 24 hours by MTT assay. Apoptosis was analysed by flocytometry. Results: The IC50 value for silver nanoparticles was 4.88 µg/ml during 24 h on AGS cell line, which was significant comparing to the control group. Also the IC50 value for silver nanoparticles in 24 hours for normal cells was 14.56 µg/ml. Apoptosis was detected in test group which was more than necrosis. A significant difference was observed between apoptosis and necrosis. Conclusion: In this study the extract of Artemisia turcomanica was used for the first time for biosynthesis of silver nanoparticles and results suggest that synthesized silver nanoparticles have cytotoxicity and apoptosis induction effect on gastric cancer cells.

Keywords

• <i>artemisia turcomanica</i>
• biological nanosilver
• cytotoxicity