A new system for multiplexed mosaic analysis of gene function in the mouse

Hongchen Cai; Monte M. Winslow

Cell Reports: Methods (Sep 2022)

A new system for multiplexed mosaic analysis of gene function in the mouse

Hongchen Cai,
Monte M. Winslow

Affiliations

Hongchen Cai: Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA
Monte M. Winslow: Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA; Corresponding author

Journal volume & issue: Vol. 2, no. 9
p. 100295

Abstract

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In a recent issue of Cell, Liu et al. present an innovative mouse model system in which Cre/lox stochastically turns on transgenic expression of one out of up to 100 sgRNAs in somatic cells, creating genetic mosaicism that enables the multiplexed assessment of gene function in vivo.

Published in Cell Reports: Methods

ISSN: 2667-2375 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Technology: Chemical technology: Biotechnology; Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.cell.com/cell-reports-methods

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