Isolation of Mouse Tumor-Infiltrating Leukocytes by Percoll Gradient Centrifugation
Ying Liu,
Keqiang Chen,
Chunyan Wang,
Wanghua Gong,
Teizo Yoshimura,
Ji Wang,
Mingyong Liu
Affiliations
Ying Liu
Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, Center of Cancer Research, National Cancer Institute-Frederick, NIH, Frederick, USA
Keqiang Chen
Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, Center of Cancer Research, National Cancer Institute-Frederick, NIH, Frederick, USA
Chunyan Wang
Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, Center of Cancer Research, National Cancer Institute-Frederick, NIH, Frederick, USA
Wanghua Gong
Basic Research Program, SAIC-Frederick, Frederick, USA
Teizo Yoshimura
Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, Center of Cancer Research, National Cancer Institute-Frederick, NIH, Frederick, USA
Ji Wang
Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, Center of Cancer Research, National Cancer Institute-Frederick, NIH, Frederick, USA
Mingyong Liu
Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, Center of Cancer Research, National Cancer Institute-Frederick, NIH, Frederick, USADepartment of Spine Surgery, Daping Hospital, Third Military Medical University, Chongqing, China
A hallmark of cancer-associated inflammation is the infiltration of leukocytes into tumors, which is believed to be recruited by chemokines. Some infiltrating leukocytes such as macrophages often promote tumor growth by producing growth-inducing and angiogenic factors. Here, we described a method of isolating tumor-infiltrating leukocytes with Percoll density gradient, because Percoll possesses a low viscosity, a low osmolarity and no toxicity to cells. Different leukocyte populations are isolated based on their density and collected at the interface between 40% and 80% discontinuous Percoll gradient.
