Identification and expression profiles of Tubby‑like proteins coding genes in walnut (Juglans regia L.) in response to stress and hormone treatments
Guoquan Zheng,
Tianle Zhang,
Jiale Liu,
Rui Yan,
Wenxiu Wang,
Ning Wang,
Faria Sundas,
Kaiyu Yang,
Qinglong Dong,
Haoan Luan,
Xuemei Zhang,
Han Li,
Suping Guo,
Guohui Qi,
Peng Jia
Affiliations
Guoquan Zheng
College of Forestry, Hebei Agricultural University, Baoding 071000, China
Tianle Zhang
College of Forestry, Hebei Agricultural University, Baoding 071000, China
Jiale Liu
College of Forestry, Hebei Agricultural University, Baoding 071000, China
Rui Yan
College of Forestry, Hebei Agricultural University, Baoding 071000, China
Wenxiu Wang
College of Forestry, Hebei Agricultural University, Baoding 071000, China
Ning Wang
College of Forestry, Hebei Agricultural University, Baoding 071000, China
Faria Sundas
Agricultural College of Guizhou University, Guizhou University, Guiyang 550025, China
Kaiyu Yang
College of Forestry, Hebei Agricultural University, Baoding 071000, China
Qinglong Dong
College of Forestry, Hebei Agricultural University, Baoding 071000, China
Haoan Luan
College of Forestry, Hebei Agricultural University, Baoding 071000, China
Xuemei Zhang
College of Forestry, Hebei Agricultural University, Baoding 071000, China
Han Li
College of Forestry, Hebei Agricultural University, Baoding 071000, China
Suping Guo
College of Forestry, Hebei Agricultural University, Baoding 071000, China
Guohui Qi
College of Forestry, Hebei Agricultural University, Baoding 071000, China; Technology Innovation Center of Hebei Province, Baoding 071000, China; Corresponding authors.
Peng Jia
College of Forestry, Hebei Agricultural University, Baoding 071000, China; Technology Innovation Center of Hebei Province, Baoding 071000, China; Corresponding authors.
Tubby-like proteins (TLPs) constitute a diverse multigene family found across a broad range of eukaryotic organisms, from single-celled to multicellular eukaryotes. Despite their importance, the existence of TLPs in walnuts (Juglans regia L.) has not been reported until now. In this study, we identified 12 members of the JrTLP gene family in the walnut genome, revealing a pattern of gene replication events. Notably, all JrTLPs exhibited a split gene structure, containing two to eight introns, and these genes could be divided into five distinct categories based on their evolutionary relationships. The majority of TLP proteins possess two signature domains: the F-box and Tubby domains. Subcellular localization prediction and experiments confirmed that JrTLP2 was located in the nucleus and membrane. Expression profiling revealed that multiple JrTLPs respond to a range of stimuli, including pathogen infection, hormonal treatment (such as auxin, ABA, MeJA, and GA), and abiotic stress conditions such as cold, heat, and salt. This study represents the first comprehensive identification of the walnut TLP family, providing valuable insights into the function and regulation of TLP in walnut and laying the foundation for future functional dissection, particularly in the context of stress tolerance.
