A Scalable and Efficient Bioprocess for Manufacturing Human Pluripotent Stem Cell-Derived Endothelial Cells

Haishuang Lin; Qian Du; Qiang Li; Ou Wang; Zhanqi Wang; Neety Sahu; Christian Elowsky; Kan Liu; Chi Zhang; Soonkyu Chung; Bin Duan; Yuguo Lei

Stem Cell Reports (Aug 2018)

A Scalable and Efficient Bioprocess for Manufacturing Human Pluripotent Stem Cell-Derived Endothelial Cells

Haishuang Lin,
Qian Du,
Qiang Li,
Ou Wang,
Zhanqi Wang,
Neety Sahu,
Christian Elowsky,
Kan Liu,
Chi Zhang,
Soonkyu Chung,
Bin Duan,
Yuguo Lei

Affiliations

Haishuang Lin: Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA
Qian Du: Department of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA
Qiang Li: Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA; Biomedical Engineering Program, University of Nebraska-Lincoln, Lincoln, NE 68588, USA
Ou Wang: Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA; Biomedical Engineering Program, University of Nebraska-Lincoln, Lincoln, NE 68588, USA
Zhanqi Wang: Department of Vascular Surgery, Beijing Anzhen Hospital of Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China
Neety Sahu: Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA
Christian Elowsky: Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68588, USA
Kan Liu: Department of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA
Chi Zhang: Department of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA
Soonkyu Chung: Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USA
Bin Duan: Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA
Yuguo Lei: Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA; Biomedical Engineering Program, University of Nebraska-Lincoln, Lincoln, NE 68588, USA; Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA; Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, USA; Corresponding author

Journal volume & issue: Vol. 11, no. 2
pp. 454 – 469

Abstract

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Summary: Endothelial cells (ECs) are of great value for cell therapy, tissue engineering, and drug discovery. Obtaining high-quantity and -quality ECs remains very challenging. Here, we report a method for the scalable manufacturing of ECs from human pluripotent stem cells (hPSCs). hPSCs are expanded and differentiated into ECs in a 3D thermoreversible PNIPAAm-PEG hydrogel. The hydrogel protects cells from hydrodynamic stresses in the culture vessel and prevents cells from excessive agglomeration, leading to high-culture efficiency including high-viability (>90%), high-purity (>80%), and high-volumetric yield (2.0 × 107 cells/mL). These ECs (i.e., 3D-ECs) had similar properties as ECs made using 2D culture systems (i.e., 2D-ECs). Genome-wide gene expression analysis showed that 3D-ECs had higher expression of genes related to vasculature development, extracellular matrix, and glycolysis, while 2D-ECs had higher expression of genes related to cell proliferation. : Endothelial cells (ECs) differentiated from human pluripotent stem cells (hPSCs) are needed in large numbers for regenerative medicine. In this article, Lei and colleagues describe a simple and innovative method for the scalable production of ECs from hPSCs. The method has potential to make ECs broadly available and affordable for various applications. Key words: human pluripotent stem cells, endothelial cells, 3D culture, thermoreversible hydrogel

Published in Stem Cell Reports

ISSN: 2213-6711 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Biology (General)
Website: http://www.cell.com/stem-cell-reports/home

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