Nature Communications (Oct 2020)

CRISPRoff enables spatio-temporal control of CRISPR editing

  • Jared Carlson-Stevermer,
  • Reed Kelso,
  • Anastasia Kadina,
  • Sahil Joshi,
  • Nicholas Rossi,
  • John Walker,
  • Rich Stoner,
  • Travis Maures

DOI
https://doi.org/10.1038/s41467-020-18853-3
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 7

Abstract

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Uncontrolled gene editing can lead to off-target effects and chromosomal translocations. Here the authors develop CRISPRoff, light degradable sgRNAs for titratable and spatially defined gene editing.