Oral Cancer Theranostic Application of FeAu Bimetallic Nanoparticles Conjugated with MMP-1 Antibody
Meng-Tsan Tsai,
Ying-Sui Sun,
Murugan Keerthi,
Asit Kumar Panda,
Udesh Dhawan,
Yung-Hsiang Chang,
Chih-Fang Lai,
Michael Hsiao,
Huey-Yuan Wang,
Ren-Jei Chung
Affiliations
Meng-Tsan Tsai
Department of Electrical Engineering, Chang Gung University, 259, Wenhua 1st Rd., Taoyuan City 33302, Taiwan
Ying-Sui Sun
School of Dental Technology, College of Oral Medicine, Taipei Medical University, 250, Wu-Hsing St., Taipei 11031, Taiwan
Murugan Keerthi
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology (Taipei Tech), 1, Sec. 3, Zhongxiao E. Rd., Taipei 10608, Taiwan
Asit Kumar Panda
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology (Taipei Tech), 1, Sec. 3, Zhongxiao E. Rd., Taipei 10608, Taiwan
Udesh Dhawan
Centre for the Cellular Microenvironment, University of Glasgow, Glasgow G12 8QQ, UK
Yung-Hsiang Chang
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology (Taipei Tech), 1, Sec. 3, Zhongxiao E. Rd., Taipei 10608, Taiwan
Chih-Fang Lai
DFON Biomedical Technology Inc., 1, Sec. 3, Zhongxiao E. Rd., Taipei 10608, Taiwan
Michael Hsiao
Genomics Research Center, Academia Sinica, 128, Sec. 2, Academia Rd., Nankang, Taipei 115, Taiwan
Huey-Yuan Wang
Department of Stomatology, MacKay Memorial Hospital, 92, Sec. 2, Zhongshan N. Rd., Taipei 10449, Taiwan
Ren-Jei Chung
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology (Taipei Tech), 1, Sec. 3, Zhongxiao E. Rd., Taipei 10608, Taiwan
Metastatic oral squamous cell carcinoma (SCC) displays a poor disease prognosis with a 5-year survival rate of 39%. Chemotherapy has emerged as the mainstream treatment against small clusters of cancer cells but poses more risks than benefits for metastatic cells due to the non-specificity and cytotoxicity. To overcome these obstacles, we conjugated antibodies specific for matrix metalloproteinase-1 (MMP-1), a prognostic biomarker of SCC, to iron–gold bimetallic nanoparticles (FeAu NPs) and explored the capability of this complex to target and limit SSC cell growth via magnetic field-induced hyperthermia. Our results showed that 4.32 ± 0.79 nm sized FeAu NPs were superparamagnetic in nature with a saturation magnetization (Ms) of 5.8 emu/g and elevated the media temperature to 45 °C, confirming the prospect to deliver hyperthermia. Furthermore, conjugation with MMP-1 antibodies resulted in a 3.07-fold higher uptake in HSC-3 (human tongue squamous cell carcinoma) cells as compared to L929 (fibroblast) cells, which translated to a 5-fold decrease in cell viability, confirming SCC targeting. Finally, upon magnetic stimulation, MMP-1-FeAu NPs conjugate triggered 89% HSC-3 cellular death, confirming the efficacy of antibody-conjugated nanoparticles in limiting SCC growth. The synergistic effect of biomarker-specific antibodies and magnetic nanoparticle-induced hyperthermia may open new doors towards SCC targeting for improved disease prognosis.
