Journal of Extracellular Vesicles (Apr 2015)

Potentials and capabilities of the Extracellular Vesicle (EV) Array

  • Malene Møller Jørgensen,
  • Rikke Bæk,
  • Kim Varming

DOI
https://doi.org/10.3402/jev.v4.26048
Journal volume & issue
Vol. 4, no. 0
pp. 1 – 8

Abstract

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Extracellular vesicles (EVs) and exosomes are difficult to enrich or purify from biofluids, hence quantification and phenotyping of these are tedious and inaccurate. The multiplexed, highly sensitive and high-throughput platform of the EV Array presented by Jørgensen et al., (J Extracell Vesicles, 2013; 2: 10) has been refined regarding the capabilities of the method for characterization and molecular profiling of EV surface markers. Here, we present an extended microarray platform to detect and phenotype plasma-derived EVs (optimized for exosomes) for up to 60 antigens without any enrichment or purification prior to analysis.

Keywords