Frontiers in Nutrition (Apr 2024)

Association between the relative abundance of phyla actinobacteria, vitamin C consumption, and DNA methylation of genes linked to immune response pathways

  • Natália Yumi Noronha,
  • Natália Yumi Noronha,
  • Isabella Harumi Yonehara Noma,
  • Rafael Fernandes Ferreira,
  • Guilherme da Silva Rodrigues,
  • Luzania dos Santos Martins,
  • Lígia Moriguchi Watanabe,
  • Marcela Augusta de Souza Pinhel,
  • Marcela Augusta de Souza Pinhel,
  • Isabelle Mello Schineider,
  • Luísa Maria Diani,
  • Daniela Carlos,
  • Carla Barbosa Nonino

DOI
https://doi.org/10.3389/fnut.2024.1373499
Journal volume & issue
Vol. 11

Abstract

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IntroductionThere is an emerging body of evidence that vitamin C consumption can modulate microbiota abundance and can also impact DNA methylation in the host, and this could be a link between diet, microbiota, and immune response. The objective of this study was to evaluate common CpG sites associated with both vitamin C and microbiota phyla abundance.MethodsSix healthy women participated in this cohort study. They were divided into two groups, according to the amount of vitamin C they ingested. Ingestion was evaluated using the 24-h recall method. The Illumina 450 k BeadChip was used to evaluate DNA methylation. Singular value decomposition analyses were used to evaluate the principal components of this dataset. Associations were evaluated using the differentially methylated position function from the Champ package for R Studio.Results and discussionThe group with higher vitamin C (HVC) ingestion also had a higher relative abundance of Actinobacteria. There was a positive correlation between those variables (r = 0.84, p = 0.01). The HVC group also had higher granulocytes, and regarding DNA methylation, there were 207 CpG sites commonly related to vitamin C ingestion and the relative abundance of Actinobacteria. From these sites, there were 13 sites hypomethylated and 103 hypermethylated. The hypomethylated targets involved the respective processes: immune function, glucose homeostasis, and general cellular metabolism. The hypermethylated sites were also enriched in immune function-related processes, and interestingly, more immune responses against pathogens were detected. These findings contribute to understanding the interaction between nutrients, microbiota, DNA methylation, and the immune response.

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