Mapping of proteomic lysate of a MCF-7 cancer cell line for the identification of potential markers for breast cancer

Abstract

Mass spectrometric mapping of proteomic lysate of a MCF-7 cancer cell line was carried out, which identified 153 proteins having molecular weights of 5000 to 630000 Da, a high proportion of which was cytoplasmic and nuclear proteins. The latter accounted for 60 % of their total number whereas the proportion of extracellular and membrane proteins constituted 13 %. After using some selection criteria to analyze the findings, the authors present a list of 31 potential biomarkers and describe 12 promising breast anticancer therapeutic targets.

Keywords

• breast cancer
• mcf-7 cancer cell line
• proteomics
• mass spectrometry
• biomarkers
• therapeutic targets