Development of a Nucleic Acid Lateral Flow Immunoassay for the Detection of Human Polyomavirus BK
Yi-Huei Huang,
Kuan-Yi Yu,
Shou-Ping Huang,
Hui-Wen Chuang,
Wen-Zhi Lin,
Juin-Hong Cherng,
Yao-Wen Hung,
Ming-Kung Yeh,
Po-Da Hong,
Cheng-Che Liu
Affiliations
Yi-Huei Huang
Biomedical Engineering Program, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 10607, Taiwan
Kuan-Yi Yu
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 11490, Taiwan
Shou-Ping Huang
Department of Physiology and Biophysics, Graduate Institute of Physiology, National Defense Medical Center, Taipei 11490, Taiwan
Hui-Wen Chuang
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 11490, Taiwan
Wen-Zhi Lin
Institute of Preventive Medicine, National Defense Medical Center, Taipei 11490, Taiwan
Juin-Hong Cherng
Department and Graduate Institute of Biology and Anatomy, National Defense Medical Center, Taipei 11490, Taiwan
Yao-Wen Hung
Institute of Preventive Medicine, National Defense Medical Center, Taipei 11490, Taiwan
Ming-Kung Yeh
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 11490, Taiwan
Po-Da Hong
Biomedical Engineering Program, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 10607, Taiwan
Cheng-Che Liu
Department of Physiology and Biophysics, Graduate Institute of Physiology, National Defense Medical Center, Taipei 11490, Taiwan
The BK virus (BKV) is an emerging pathogen in immunocompromised individuals and widespread in the human population. Polymerase chain reaction is a simple and highly sensitive method for detecting BKV, but it is time consuming and requires expensive instruments and expert judgment. The lateral flow assay, a rapid, low-cost, minimal-labor, and easy-to-use diagnostic method, was successfully applied for pathogen detection. In this study, we used oligonucleotide probes to develop a simple and rapid sandwich-type lateral flow immunoassay for detecting BKV DNA within 45 minutes. The detection limit for the synthetic single-stranded DNA was 5 nM. The specificity study showed no cross-reactivity with other polyomaviruses, such as JC virus and simian virus 40. For the Escherichia coli containing BKV plasmid cultured samples, the sensitivity was determined to be 107 copies/mL. The approach offers great potential for BKV detection of various target analytes in point-of-care settings.
