Heterologous expression and functional characterization of a GH10 endoxylanase from Aspergillus fumigatus var. niveus with potential biotechnological application

Josman Velasco; Bianca Oliva; Evandro José Mulinari; Leidy Patricia Quintero; Awana da Silva Lima; Aline Larissa Gonçalves; Thiago Augusto Gonçalves; André Damasio; Fabio Marcio Squina; Adriane Maria Ferreira Milagres; Asmaa Abdella; Mark R. Wilkins; Fernando Segato

Biotechnology Reports (Dec 2019)

Heterologous expression and functional characterization of a GH10 endoxylanase from Aspergillus fumigatus var. niveus with potential biotechnological application

Josman Velasco,
Bianca Oliva,
Evandro José Mulinari,
Leidy Patricia Quintero,
Awana da Silva Lima,
Aline Larissa Gonçalves,
Thiago Augusto Gonçalves,
André Damasio,
Fabio Marcio Squina,
Adriane Maria Ferreira Milagres,
Asmaa Abdella,
Mark R. Wilkins,
Fernando Segato

Affiliations

Josman Velasco: Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Lorena, SP, Brazil
Bianca Oliva: Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Lorena, SP, Brazil
Evandro José Mulinari: Department of Physics and Applied Sciences, São Carlos Institute of Physics, University of São Paulo, São Carlos, SP, Brazil
Leidy Patricia Quintero: Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Lorena, SP, Brazil
Awana da Silva Lima: Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Lorena, SP, Brazil
Aline Larissa Gonçalves: Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Lorena, SP, Brazil
Thiago Augusto Gonçalves: Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil; Programa de Processos Tecnológicos e Ambientais, Universidade de Sorocaba, Sorocaba, SP, Brazil
André Damasio: Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil
Fabio Marcio Squina: Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil; Programa de Processos Tecnológicos e Ambientais, Universidade de Sorocaba, Sorocaba, SP, Brazil
Adriane Maria Ferreira Milagres: Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Lorena, SP, Brazil
Asmaa Abdella: Department of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA; Industrial Agricultural Products Center, University of Nebraska-Lincoln, Lincoln, NE, USA; Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt
Mark R. Wilkins: Department of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA; Industrial Agricultural Products Center, University of Nebraska-Lincoln, Lincoln, NE, USA; Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE, USA
Fernando Segato: Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Lorena, SP, Brazil; Corresponding author at: Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Estrada Municipal do Campinho, s/n, Lorena, SP, 12602-810, Brazil.

Journal volume & issue: Vol. 24

Abstract

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Xylanases decrease the xylan content in pretreated biomass releasing it from hemicellulose, thus improving the accessibility of cellulose for cellulases. In this work, an endo-β-1,4-xylanase from Aspergillus fumigatus var. niveus (AFUMN-GH10) was successfully expressed. The structural analysis and biochemical characterization showed this AFUMN-GH10 does not contain a carbohydrate-binding module. The enzyme retained its activity in a pH range from 4.5 to 7.0, with an optimal temperature at 60 °C. AFUMN-GH10 showed the highest activity in beechwood xylan. The mode of action of AFUMN-GH10 was investigated by hydrolysis of APTS-labeled xylohexaose, which resulted in xylotriose and xylobiose as the main products. AFUMN-GH10 released 27% of residual xylan from hydrothermally-pretreated corn stover and 14% of residual xylan from hydrothermally-pretreated sugarcane bagasse. The results showed that environmentally friendly pretreatment followed by enzymatic hydrolysis with AFUMN-GH10 in low concentration is a suitable method to remove part of residual and recalcitrant hemicellulose from biomass. Keywords: Endoxylanase, Filamentous fungi, Corn stover, Sugarcane bagasse, Xylooligosaccharides

Published in Biotechnology Reports

ISSN: 2215-017X (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://www.journals.elsevier.com/biotechnology-reports/

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