Assembly of anhydrobiotic midge Polypedilum vanderplanki genome using Illumina and PacBio data

Abstract

A completely new version of assembly of the African anhydrobiotic midge Polypedilum vanderplanki genome derived by deep DNA sequencing of the Pv11 cell line has been discussed. The input data include paired-end and mate-paired reads with various in­sert sizes supplemented with ultra-long reads of Pacific Biosciences platform sequencing. We have shown that the resulting set of scaffolds has higher continuity and completeness metrics and, besides, can provide more correct predictions of coding sequences as compared to the previous assembly version, which has been demonstrated based on heat-shock proteins HSP20 and HSP70.

Keywords

• polypedilum vanderplanki
• anhydrobiosis
• dna sequencing
• genome assembly