Journal of Fungi (Dec 2019)

Chromosome-Level Comprehensive Genome of Mangrove Sediment-Derived Fungus <i>Penicillium variabile</i> HXQ-H-1

  • Ling Peng,
  • Liangwei Li,
  • Xiaochuan Liu,
  • Jianwei Chen,
  • Chengcheng Shi,
  • Wenjie Guo,
  • Qiwu Xu,
  • Guangyi Fan,
  • Xin Liu,
  • Dehai Li

DOI
https://doi.org/10.3390/jof6010007
Journal volume & issue
Vol. 6, no. 1
p. 7

Abstract

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Penicillium is an ascomycetous genus widely distributed in the natural environment and is one of the dominant fungi involved in the decomposition of mangroves, which can produce a variety of antitumor compounds and bioactive substances. However, in mangrove ecosystems there is no complete genome in this genus. In this study, we isolated a fungus strain named Penicillium variabile HXQ-H-1 from coast mangrove (Fujian Province, China). We generated a chromosome-level genome with total size of 33.32 Mb, scaffold N50 of 5.23 Mb and contig N50 of 96.74 kb. Additionally, we anchored about 95.91% assembly sequences into the longest seven scaffolds, and predicted 10,622 protein-coding genes, in which 99.66% could be annotated by eight protein databases. The secondary metabolites analysis reveals the strain has various gene clusters involving polyketide synthase (PKS), non-ribosomal peptide synthetase (NRPS) and terpene synthase that may have a largely capacity of biotechnological potential. Comparison genome analysis between Penicillium variabile and Talaromyces islandicus reveals a small difference in the total number of genes, whereas HXQ-H-1 has a higher gene number with COG functional annotation. Evolutionary relationship of Penicillum based on genome-wide data was carried out for the first time, showing the strain HXQ-H-1 is closely related to Talaromyces islandicus. This genomic resource may provide a new resource for development of novel bioactive antibiotics, drug candidates and precursors in Penicillium variabile.

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