Identification and analysis of senescence-related genes in caudal fin cells of triploid crucian carp

Canhui Jiang; Zhen Huang; Lingwei Tang; Fangyuan Peng; Yamei Xiao

Reproduction and Breeding (Dec 2023)

Identification and analysis of senescence-related genes in caudal fin cells of triploid crucian carp

Canhui Jiang,
Zhen Huang,
Lingwei Tang,
Fangyuan Peng,
Yamei Xiao

Affiliations

Canhui Jiang: University of Adelaide, South Australia, 5005, Australia
Zhen Huang: State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, 410081, China; College of Life Sciences, Hunan Normal University, Changsha, 410081, China
Lingwei Tang: State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, 410081, China; College of Life Sciences, Hunan Normal University, Changsha, 410081, China
Fangyuan Peng: State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, 410081, China; College of Life Sciences, Hunan Normal University, Changsha, 410081, China
Yamei Xiao: State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, 410081, China; College of Life Sciences, Hunan Normal University, Changsha, 410081, China; Corresponding author. State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha, 410081, China.

Journal volume & issue: Vol. 3, no. 4
pp. 169 – 175

Abstract

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This research aims to identify the hub genes associated with the senescence of triploid caudal fin cells. Transcriptomic data are obtained from the high and low generation (P6, P60) of triploid crucian carp caudal fin cells by high-throughput sequencing technology. Initially, all differential genes between the high and low generations are screened, yielding 4140 significantly upregulated genes and 3724 significantly downregulated genes. Subsequently, an aging gene set containing 950 genes is downloaded from the CellAge database to extract the differentially expressed genes associated with caudal fin cell aging, totaling 29 genes. GO and KEGG enrichment analyses are performed on these 29 aging differential genes. The GO analysis shows enrichment mainly in cellular processes related to aging, such as regulation of cell division, chromatin organization, cell cycle regulation. KEGG analysis reveals that the 29 aging-related genes are primarily involved in cell cycle and cellular senescence pathways. A PPI network of aging-related genes is constructed using the STRING database and Cytoscape software. Top-ranked genes were identified by using Degree, MCC, MNC, and Closeness algorithms in the Cytohubba plugin in Cytoscape, resulting in hub genes EZH2, JUN, MYD88, RBL2, BMP4, CCND1, NFKB2, MMP9. Lastly, qRT-PCR validation of these eight hub genes further confirmed the involvement of four genes: EZH2, RBL2, BMP4, and CCND1. The hub gene screened in this study may become a potential biomarker of fish caudal fin cell senescence, which provides a valuable experimental basis for the senescence of fish caudal fin cells, especially the senescence of caudal fin cells in polyploid fish, and the reproduction and breeding improvement of polyploid fish. It also provides meaningful data for elucidating the molecular mechanism of polyploid formation in animals, as well as the formation of aging and tumour in human beings.

Published in Reproduction and Breeding

ISSN: 2667-0712 (Online)
Publisher: KeAi Communications Co. Ltd.
Country of publisher: China
LCC subjects: Science: Biology (General): Genetics; Science: Biology (General): Reproduction; Science: Physiology: Animal biochemistry
Website: https://www.keaipublishing.com/en/journals/reproduction-and-breeding/

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