Conservation of grape genetic resources in the system in vitro

Abstract

Conservation of grape genetic resources in the system in vitro can be a contribution to ampelographic collections, and a source of their replenishment with healthy planting material. The goal of the study is to create a vegetative in vitro collection of plants of promising grape varieties and clones, optimize the conditions for maintaining samples. The basis for obtaining, cultivating, clonal micro-propagating of grape plants is the research and development of the Institute Magarach. At the moment, the collection is represented by 144 samples of grape varieties and clones. Samples of the collection are kept in a state of active growth, slow growth and true dormancy. Previous studies followed to establishment of five modes to maintain grape plants in the system in vitro. Changes in the parameters of individual factors affecting morphogenesis made it possible to slow down growth processes and reduce the number of re-plantings. The most promising mode to conserve samples is the fifth one (this method is patented). As a result of the research carried out in the Institute Magarach, a vegetative collection of plants in vitro of promising grape varieties and clones was created. It includes Crimean autochthonous varieties and varieties of the Institute Magarach breeding. The developed modes of collection conservation allow slowing down growth processes and maintaining plants in a state of true dormancy without re-planting for one to two years.