Fiyz̤ (Oct 2022)
The effect of high intensity interval training and caloric restriction on inflammatory factors and periplipin3 visceral adipose tissue in male Wistar rats with type 2 diabetes
Abstract
Background: There is ample evidence in the study of the association of inflammatory reactions with the pathogenesis and complications of diabetes, especially hyperlipidemia and atherosclerosis. This study aimed to the effect of high intensity interval training and caloric restriction on inflammatory factors and PLIN3 visceral adipose tissue in male diabetic rats. Materials and Methods: 32 diabetic male Wistar rats was randomly divided into three subgroups: HIIT, control and caloric restriction. In the caloric restriction group, 40% of the calorie intake was restricted and other groups received the standard food they needed freely. Relative protein expression of PLIN3 was performed using western blot technique and TNF-α and IL-6 by ELISA. Data were analyzed by one-way ANOVA and Tukey's post hoc test. Result: There was a significant difference between the mean of PLIN3, IL-6 and TNF-α in the three groups (P=0.001). The results of post hoc test showed a significant increase in PLIN3 and a significant decrease in IL-6 and TNF-α in the caloric restriction group and intense intermittent exercise compared to the diabetic control group (P=0.001). Conclusion: HIIT and caloric restriction may increase lipid oxidation by increasing PLIN3 expression. Considering the role of TNF-α and IL-6 in increasing insulin resistance and type 2 diabetes, reducing these factors during the present study may be helpful in preventing these metabolic diseases.
