First cytogenomic characterization of murine cutaneous sarcoma cell line TSC2ang1

Abstract

The murine cutaneous sarcoma cell line TSC2ang1 has been established in 1999 and recently been suggested to be a model for tuberous sclerosis-associated skin lesions/ hamartomas. This suggestion was founded in first place on the fact that the cell line has been established from tumor arising in a heterozygote tsc2-gene knockout mouse. Here, as the genetic characteristics of TSC2ang1 have never been determined in detail before, chromosome microarray and multicolor banding-based molecular cytogenetics were done. A near tetraploid karyotype with 69 to 77 chromosomes per cell, and two equally sized subclones were characterized for TSC2ang1. Into the human genome translated results of identified gains and losses, and a literature review showed that TSC2ang1 is rather suited as a sarcoma model than for tuberous sclerosis-associated hamartomas.

Keywords

• murine tumor cell line
• sarcoma
• tuberous sclerosis
• hamartomas
• molecular cytogenetics