Biological Behavior of Bioactive Glasses SinGlass (45S5) and SinGlass High (F18) in the Repair of Critical Bone Defects
Dayane Maria Braz Nogueira,
Marcelie Priscila de Oliveira Rosso,
Paulo Sérgio da Silva Santos,
Manoel Damião Sousa-Neto,
Alice Corrêa Silva-Sousa,
Cleverson Teixeira Soares,
Carlos Henrique Bertoni Reis,
Jéssica de Oliveira Rossi,
Cleuber Rodrigo de Souza Bueno,
Daniela Vieira Buchaim,
Rogério Leone Buchaim,
Mariana Schutzer Ragghianti Zangrando
Affiliations
Dayane Maria Braz Nogueira
Department of Anatomy, Faculty of Higher Education of the Interior of São Paulo (FAIP), Marília 17512-130, Brazil
Marcelie Priscila de Oliveira Rosso
Department of Physiotherapy, Physical Education, and Biomedicine, Estácio de Sá School of Ourinhos (FAESO), Ourinhos 19907-510, Brazil
Paulo Sérgio da Silva Santos
Department of Surgery, Stomatology, Pathology, and Radiology, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, Brazil
Manoel Damião Sousa-Neto
Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, Brazil
Alice Corrêa Silva-Sousa
Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, Brazil
Cleverson Teixeira Soares
Lauro de Souza Lima Institute (ILSL), Anatomical Pathology Laboratory of Bauru (ANATOMED), Bauru 17034-971, Brazil
Carlos Henrique Bertoni Reis
Beneficent Hospital (HBU), University of Marilia (UNIMAR), Marilia 17525-160, Brazil
Jéssica de Oliveira Rossi
Occupational Medicine, Marilia School of Medicine (FAMEMA), Marilia 17519-030, Brazil
Cleuber Rodrigo de Souza Bueno
Anatomy Department, Faculty of Dentistry, University Center of Adamantina (FAI), Adamantina 17800-000, Brazil
Daniela Vieira Buchaim
Anatomy Department, Medical School, University Center of Adamantina (FAI), Adamantina 17800-000, Brazil
Rogério Leone Buchaim
Graduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ/USP), Sao Paulo 05508-270, Brazil
Mariana Schutzer Ragghianti Zangrando
Department of Prosthodontics and Periodontics, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, Brazil
This study evaluated the osteogenic potential of the bioactive glasses SinGlass (45S5) and SinGlass High (F18) in regenerating critical bone defects in rat calvaria. Both biomaterials promoted new bone formation around the particles, with the SinGlass High (F18) group exhibiting a higher rate of bone maturation. Histomorphological and birefringence analyses revealed better organization of the newly formed bone in the biomaterial-treated groups, and immunohistochemistry indicated the expression of osteogenic markers such as osteocalcin, immunostaining for bone morphogenetic protein 2 (BMP 2), and immunostaining for bone morphogenetic protein 4 (BMP 4). Microtomography computadorized (Micro-CT) revealed centripetal bone formation in both groups, with greater integration of the particles into the surrounding bone tissue. The superior performance of SinGlass High (F18) was attributed to its higher potassium and magnesium content, which enhance osteoconductivity. After 42 days, the SinGlass High (F18) group showed the highest percentage of new bone formation, in line with previous studies. Although our results are promising, the limited follow-up period and use of a single animal model highlight the need for further research to validate clinical applicability. SinGlass High (F18) appears to be a viable alternative to autografts in bone repair, with potential to improve tissue integration and accelerate recovery.
