Interlaboratory validation data on real-time polymerase chain reaction detection for unauthorized genetically modified papaya line PRSV-YK
Kosuke Nakamura,
Kazunari Kondo,
Hiroshi Akiyama,
Takumi Ishigaki,
Akio Noguchi,
Hiroshi Katsumata,
Kazuto Takasaki,
Satoshi Futo,
Kozue Sakata,
Nozomi Fukuda,
Junichi Mano,
Kazumi Kitta,
Hidenori Tanaka,
Ryo Akashi,
Tomoko Nishimaki-Mogami
Affiliations
Kosuke Nakamura
National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan; Corresponding authors.
Kazunari Kondo
National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan; Corresponding authors.
Hiroshi Akiyama
National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan
Takumi Ishigaki
National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan
Akio Noguchi
National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan
Hiroshi Katsumata
FASMAC CO., LTD., 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan
Kazuto Takasaki
FASMAC CO., LTD., 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan
Satoshi Futo
FASMAC CO., LTD., 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan
Kozue Sakata
National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan
Nozomi Fukuda
National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan
Junichi Mano
Analytical Science Division, National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan
Kazumi Kitta
Analytical Science Division, National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan
Hidenori Tanaka
Graduate School of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan
Ryo Akashi
Graduate School of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan
Tomoko Nishimaki-Mogami
National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan
This article is referred to research article entitled “Whole genome sequence analysis of unidentified genetically modified papaya for development of a specific detection method” (Nakamura et al., 2016) [1].Real-time polymerase chain reaction (PCR) detection method for unauthorized genetically modified (GM) papaya (Carica papaya L.) line PRSV-YK (PRSV-YK detection method) was developed using whole genome sequence data (DDBJ Sequenced Read Archive under accession No. PRJDB3976). Interlaboratory validation datasets for PRSV-YK detection method were provided. Data indicating homogeneity of samples prepared for interlaboratory validation were included. Specificity and sensitivity test data for PRSV-YK detection method were also provided. Keywords: Genetically modified, Real-time PCR, Carica papaya L., Validation data
