Rutin Alleviates Zearalenone-Induced Endoplasmic Reticulum Stress and Mitochondrial Pathway Apoptosis in Porcine Endometrial Stromal Cells by Promoting the Expression of Nrf2
Chuangjiang Chen,
Chenlong Wang,
Hui Jiang,
Mengya Wang,
Sajid Ur Rahman,
Changjiang Chen,
Hongyan Ding,
Chang Zhao,
Wanyue Huang,
Xichun Wang
Affiliations
Chuangjiang Chen
College of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, China
Chenlong Wang
College of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, China
Hui Jiang
College of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, China
Mengya Wang
College of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, China
Sajid Ur Rahman
School of Public Health and Emergency Management, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China
Changjiang Chen
Huangyuan County Animal Husbandry and Veterinary Station, Huangyuan County Agriculture and Rural affairs Bureau, Xining 812100, China
Hongyan Ding
Anhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering, Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230001, China
Chang Zhao
College of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, China
Wanyue Huang
College of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, China
Xichun Wang
College of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, China
Zearalenone (ZEA) is a mycotoxin commonly found in moldy cereals and has a range of toxic effects that have seriously affected animal husbandry. Rutin, a natural flavonoid with antioxidant activities, has been studied for its potential involvement in mitigating ZEA-induced apoptosis in porcine endometrial stromal cells (ESCs) and its potential molecular mechanism, particularly concerning the expression of Nrf2. This study investigates the molecular pathways by which rutin alleviates ZEA-induced ESC apoptosis, focusing on the role of Nrf2. Experimental data reveal that ZEA suppresses Nrf2 nuclear translocation and reduces mitochondrial membrane potential (MMP), leading to oxidative stress, endoplasmic reticulum stress (ERS), and mitochondrial pathway-driven apoptosis. Notably, rutin mitigates ZEA-induced apoptosis through Nrf2 activation. These findings highlight Nrf2 as a critical factor in rutin’s protective effects against ZEA-induced apoptosis, offering valuable insights for the clinical prevention and treatment of ZEA toxicity.
