PHYLOGENETIC ANALYSIS OF HUMAN INFLUENZA VIRUSES А(H3N2), ISOLATED IN UKRAINE DURING THE 2011–2012 EPIDEMIC SEASON

Abstract

Aim. To perform the phylogenetic analysis of segments, encodes hemagglutinin (HA) and neuraminidase (NA) of A (H3N2) influenza viruses, isolated in Ukraine during the 2011–2012 epidemic season. Methods. In this study there were used the real-time polymerase chain reaction (realtime RT-PCR), sequencing and phylogenetic analysis methods. Results. The genetic segments HA and NA of Ukrainian influenza isolates of A (H3N2) influenza were analyzed. Appropriate phylogenetic trees were constructed by Neighbor-Joining method using the Kimura 2-parameter model. The statistical evaluation of phylogenetic analysis has been performed by uping the bootstrap test (1000 replicates). The genetic similarity of ukrainian isolates, vaccine strain and viruses all over the world were analyzed. All genetic groups of Victoria/208 cluster included our isolates were described. The key mutations in amino acid sequences in HA and NA proteins were identified. Conclusions. The multiple ways of entering the epidemic strains of influenza A (H3N2) viruses 2011–2012 season in Ukraine were identified as well. The mutations in hemagglutinin of Ukrainian isolates were located in highly variable regions and weren’t associated with significant changes in viruses. No selection of resistant mutations (E119V, E119V + I222V, R292K and N294S) to oseltamivir and zanamivir in NA proteins was observed.

Keywords

• віруси грипу a(h3n2)
• нуклеотидні послідовності
• філогенетичний аналіз