Journal of Health Science and Medical Research (JHSMR) (Jun 2016)

Detection of Clinically Significant Minor Blood Group Antigens of Blood Donors in Songklanagarind Hospital

  • Darinnat Buathong,
  • Sarunya Hassarin

Journal volume & issue
Vol. 34, no. 3
pp. 109 – 118

Abstract

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Objective: The purpose of this study was to organize the data of blood donors’ antigens into a database for providing compatible blood for patients, especially those who are activated to produce antibodies. Material and Methods: Three hundred blood samples obtained from volunteer blood donors who had donated at least 3 times from the Blood Bank and Transfusion Medicine Unit of Songklanagarind Hospital were studied for antigen in the Rh, MNSs, P, Lewis, Kidd, Kell and Duffy Systems. Each sample was tested by gel test using neutral and LISS/Coombs gel of Bio-Rad Company. Results: The data of 300 donors ABO-typed, blood group 0 was the most common (40.38%) followed by group B (30.77%), group A (17.31%) and group AB (7.3%). In this study; Rh (D+) was found in sample 100%, C(+) 94.23%, E(+) 38.04%, c(+) 47.87% and e(+) 96.00%. The most common phenotype was CCDee (50.13%), which was similar to other studies. Among other minor blood group were as follow: MNSs, the most common phenotype was MNss (46.33 %) and Mia (+) (9.80%). P system, 35% were positive with anti-P1. In the Lewis, the incidence of Le (a-b-) was 12.28% which is similar with other findings in Thai population. Kidd system, Jk (a-b-) was not found, which is considered a rare phenotype among Thai people. For the Kell system, kk was the most common phenotype (99.50%). Duffy system, Fya and Fy (a+b-) is the most common antigen and phenotype in this study which was similar to other studies. Conclusion: In addition to antigens in the ABO system, red blood cell antigens detected in blood donors were consistent with antigens in the database of the Thai population. Antigen data of blood donors can be to form the database for providing compatible blood for patients. Moreover, antigen data is also useful for providing safely compatible blood immediately for patients who have been activated to produce antibodies.

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