Journal of Nephropharmacology (Jan 2019)

The protective effects of vasopressin on experimental testicular torsion-detorsion in male rats

  • Amin Hasanvand,
  • Fariba Ahmadizar,
  • Sadegh Ebrahimi-Ghalaei,
  • Fatemeh Beyranvand,
  • Reza Mohammadrezaei Khorramabadi,
  • Peyman Amanolahi Baharvand,
  • Abolfazl Abbaszadeh

DOI
https://doi.org/10.15171/npj.2019.10
Journal volume & issue
Vol. 8, no. 1
pp. 10 – 10

Abstract

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Introduction: Vasopressin is a potent vasoconstrictor. Vasopressin can reduce damages caused by ischemia-reperfusion. Objectives: The aim of this study was to evaluate the reducing effect of vasopressin on the damage caused by testicular torsion-detorsion in male rats. Materials and Methods: Fifty male rats were used in this study. The rats were divided randomly into five groups (10 per group) as follows; 1) control group (without surgery), 2) sham group (surgery without torsion-detorsion), 3) torsion-detorsion surgery without any treatment, 4) torsion-detorsion surgery with vasopressin treatment (0.1 μg/kg), 5) torsion-detorsion surgery with vasopressin treatment (0.2 μg/kg). After that, the rats were killed and subsequently the biochemical parameters were measured by commercial kits. Histological changes were assessed by H&E staining. Results: Using Kruskal Wallis test, we observed that the changes in the tissues of seminiferous tubes, testosterone levels, glutathione (GSH) and thioredoxin enzymes in the studied groups (control, sham, torsion-detorsion, torsion-detorsion with 0.1 μg/kg, torsion-detorsion with 0.2 μg/kg) were statistically significant. Conclusion: Intracellular mechanisms of vasopressin involved in the regulation of protective mechanism and can reduce damages caused by ischemia-reperfusion. Vasopressin improves the process of changes in the testicular seminiferous tubes of rats with torsion-detorsion. Moreover, it also increases serum levels of testosterone, GSH and thioredoxin enzymes compared to the torsiondetorsion group

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