MATEC Web of Conferences (Jan 2018)

Influence of the combination of last sense codon and stop codon on expression efficiency of green fluorescent protein gene in Escherichia coli when the expression vector pKK223-3

  • Zhang Xing,
  • Fei Aiping,
  • Wang Yong,
  • Fang Zhigang,
  • Teng Yingxue,
  • Hao Xiaoliang

DOI
https://doi.org/10.1051/matecconf/201823804003
Journal volume & issue
Vol. 238
p. 04003

Abstract

Read online

In this paper, the influence of the combination of last sense codon and stop codon on expression efficiency was studied. By our study, for the last sense codon CCG, the RFI of GFP(CCG) was 2.1 fold when the stop codon was UAA, but in comparison, the RFI was 1.1 fold when the stop codon was changed from UAA to UAG. For last sense codon TAG, the RFI of GFP(TAG) with the stop codon UAG was stronger than that with the stop codons UAA and UGA.