The evaluation of healing effect of triclosan and flurbiprofen-loaded nanogels in experimental periodontitis in rats by morphometric analysis

Abstract

Purpose: To evaluate therapeutic effectiveness of antibacterial triclosan (TCS) and anti-inflammatory flurbiprofen (FLB)-loaded nanogels system in ligature-induced experimental periodontitis in rats. Methodology: A total of 72 Sprague–Dawley rats were used in this study. Four groups (n = 18 each) were randomly created: Group 1 – neither subjected to experimental periodontitis nor to any treatment; Group 2 – subjected to experimental periodontitis but not treated; Group 3 – subjected to experimental periodontitis and then treated with the developed nanogels; Group 4 – subjected to experimental periodontitis and then placed on a mixture of pure TCS and FLB treatment. The experimental periodontitis was induced on the lower incisors by applying a ligature which was kept for 14 days. Treatment was done for 7 days, and sampling was done at 7, 14, and 28 day of the post-induction experimental period. Morphometric analysis was conducted to assess the clinical outcomes and healing effect. Results: The morphometric findings showed that the group treated with the developed TCS and FLB-loaded nanogels recovered better and faster than a mixture of pure TCS and FLB. At 28 day of the experimental period, there was no significant difference (p > 0.05) between the baseline control group and the nanogels treated group. Conclusions: The developed TCS and FLB-loaded nanogels was found to be effective in the treatment of experimental periodontitis in rats. The used experimental periodontitis model was found to be simple and easily reproducible.

Keywords

• Experimental periodontitis
• Morphometric analysis
• Rat incisors
• Nanogels
• Triclosan
• Flurbiprofen