Effect of the Adiantum capillus-veneris Extract on Immunohistochemical Expression of Lung Heat-Shock Proteins in Rats Exposed to Hypoxia

Abstract

Background: Despite the traditional use of the Adiantum capillus-veneris plant, there is little evidence of its clinical effects. Objective: The purpose of the present study was to investigate the effect of the Adiantum capillus-veneris extract on Immunohistochemical expression of Lung heat-Shock Proteins in rats exposed to hypoxia. Methods: Twenty-four male Wistar rats (control: 8, training-hypoxia: 8, training-hypoxia-supplement: 8) without clinically evident disease were used. The rats were exposed to hypoxia environment for 3 week following 6 weeks interval training. Tweleve of the experimental samples were taken 500 ml Adiantum capillus-veneris extract per body weight (kg) in during exposure to hypoxia environment. Finally lung tissue was removed for immunohistochemistry tests of HSP70 and HSP90. To analyze of data, ANOVA test was used (α≤0.05). Results: Expression of HSP70 and HSP90 increased significantly in hypoxia group comparison with the control group (P≤0.05). Expression of HSP70 and HSP90 protein decreased significantly in the Supplement hypoxia group comparison with the hypoxia group (P≤0.05). Conclusion: Reduction effects of Adiantum capillus-veneris extract on expression of the parenchyma lung heat- shock protein in hypoxia conditions was observed that probably indicate decreased oxidative stress in the lung.

Keywords

• <i>adiantum capillus-veneris</i>
• heat shock protein
• hypoxia
• lung