Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana
Yanrong You,
Chunpeng An,
Chuanyou Li
Affiliations
Yanrong You
State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Innovation Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaCAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China
Chunpeng An
State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Innovation Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaCAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China
Chuanyou Li
State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Innovation Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaCAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China
Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, we describe a protocol for insect feeding assays with Spodoptera exigua lavae using model host plant Arabidopsis thaliana, which is simple and easy to conduct, and can be used to evaluate the effect of host genes on insect growth and thus to study plant resistance to chewing insects.
