Nature Communications (Dec 2021)

A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium

  • Franka H. van der Linden,
  • Eike K. Mahlandt,
  • Janine J. G. Arts,
  • Joep Beumer,
  • Jens Puschhof,
  • Saskia M. A. de Man,
  • Anna O. Chertkova,
  • Bas Ponsioen,
  • Hans Clevers,
  • Jaap D. van Buul,
  • Marten Postma,
  • Theodorus W. J. Gadella,
  • Joachim Goedhart

DOI
https://doi.org/10.1038/s41467-021-27249-w
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 13

Abstract

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Currently, genetically encoded calcium indicators are not suitable for direct quantification. Here the authors engineer a fluorescence lifetime imaging calcium biosensor, Turquoise Calcium Fluorescence LIfeTime Sensor (Tq-Ca-FLITS), and measure intracellular calcium concentrations in human-derived organoids.